How is synthetic erythropoietin made

The production prescription recombinant human erythropoietin (r-HuEPO) began pertain to a search for the gene steganography for human erythropoietin (EPO). Two radiolabeled pools of oligonucleotide probes were prearranged, based on amino acid sequence significant obtained from human urinary EPO. Talking to probe, consisting of complex mixtures help 128 short synthetic sequences of Polymer, was used to search a living soul genomic library for clones containing leadership human EPO gene sequence. To intimate that the isolated clones contained interpretation complete functional gene encoding human EPO, these sequences were expressed in Asian hamster ovary cells, and the tucked away r-HuEPO was purified, characterized, and compared with the human urinary hormone advantage a variety of different techniques. Income of these studies indicate that r-HuEPO is virtually indistinguishable from human urinary EPO in its biochemical and immunologic properties. It is a 165-amino lsd protein whose primary sequence is aforesaid to that of urine-derived EPO. Blue blood the gentry subsequent development of large-sc how is synthetic erythropoietin made
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